Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies)
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Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies). / Stålbrand, H.; Lundqvist, J.; Andersson, A.; Hägglund, P.; Anderson, L.; Tjerneld, F.; Jacobs, A.; Teleman, A.; Dahlman, O.; Palm, M.; Zacchi, G.
Henicelluloses: Science and Technology. ed. / Paul Gatenholm; Maija Tenkanen. 2004. p. 66-78 (A C S Symposium Series, Vol. 864).Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review
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TY - GEN
T1 - Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies)
AU - Stålbrand, H.
AU - Lundqvist, J.
AU - Andersson, A.
AU - Hägglund, P.
AU - Anderson, L.
AU - Tjerneld, F.
AU - Jacobs, A.
AU - Teleman, A.
AU - Dahlman, O.
AU - Palm, M.
AU - Zacchi, G.
PY - 2004/12/1
Y1 - 2004/12/1
N2 - Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190° C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard molecules were prepared using SEC. The structure of the AcGGM was determined using 1H-NMR. The enzymatic hydrolysis of AcGGM was studied using β-mannanase and α-galactosidase.
AB - Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190° C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard molecules were prepared using SEC. The structure of the AcGGM was determined using 1H-NMR. The enzymatic hydrolysis of AcGGM was studied using β-mannanase and α-galactosidase.
M3 - Article in proceedings
AN - SCOPUS:38849099768
SN - 9780841238428
T3 - A C S Symposium Series
SP - 66
EP - 78
BT - Henicelluloses
A2 - Gatenholm, Paul
A2 - Tenkanen, Maija
ER -
ID: 240161930